Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role

ncrease in erythrocyte aggregation (EA) is pathognomonic for rheumatoid arthritis (RA), and its estimation through erythrocyte sedimentation rate (ESR) is part of DAS 28-4 activity diagnosis, with low correlation with EA and that does not discriminate the contribution of cell factors that increase a...

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Authors: Luquita, A., Urli, L., Svetaz, M. J., Gennaro, Ana Maria, Volpintesta, R., Palatnik, S., Rasia, M.
Format: article
Status:Published version
Publication Date:2009
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/17122
Online Access:http://hdl.handle.net/11336/17122
Access Level:Open access
Keyword:Rheumatoid Arthritis Activity
Erythrocyte Aggregation
Membrane Fluidity
Erythrocyte Deformability
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
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repository_id_str
spelling Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s roleLuquita, A.Urli, L.Svetaz, M. J.Gennaro, Ana MariaVolpintesta, R.Palatnik, S.Rasia, M.Rheumatoid Arthritis ActivityErythrocyte AggregationMembrane FluidityErythrocyte Deformabilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1ncrease in erythrocyte aggregation (EA) is pathognomonic for rheumatoid arthritis (RA), and its estimation through erythrocyte sedimentation rate (ESR) is part of DAS 28-4 activity diagnosis, with low correlation with EA and that does not discriminate the contribution of cell factors that increase aggregation. Objective: To analyse cell and plasma factors that might be involved in EA increase, to understand how RA affects blood components, thus modifying blood fluid behavior. Methodology: One hundred women presenting active RA were compared with age-matched controls (C). EA was measured by transmitted light, obtaining two parameters: 2k2n0, characterizing the aggregation process kinetics and s0/n0, estimating aggregates size. Cell factors assays: erythrocyte deformability, by filtration through nucleopore membranes, cell shape, by microscopy, and membrane fluidity by EPR. Plasma: total proteins and CRP, albumin, fibrinogen (Fb), by gravimetry, and IgG and IgM by single radial immuno-diffusion. Results: AR and C (x ± SE). 2k2n0: 31.83 ± 2.84, 23.75 ± 1.91; s0/n0: 0.92 ± 0.05, 0.87 ± 0.04. Rigidity index (RI): 14.79 ± 4.71, 6.92 ± 1.31. Morphological index: 0.28 ± 0.03, 0.30 ± 0.05, n.s. Fb (mg/dl): 382 ± 80, 299 ± 70. IgG (mg/dl): 1580 ± 219, 1296 ± 158; IgM (mg/dl) 233 ± 28, 183 ± 23; albumin (g/dl) 3.84 ± 0.44, 3.77 ± 0.51 n.s. p < 0.05 accepted. Correlations: 2k2n0 vs. Fb r = 0.66; s0/n0 vs. Fb r = 0.51; 2k2n0 vs. Igs r = 0.65; s0/n0 vs. Igs r = 0.56. 2k2n0 vs. RI r = -0.59; s0/n0 vs. RI = -0.52, p < 0.05. Conclusions: Plasma factors, Igs and Fb increased aggregation, since RI is altered, this reduces the process efficiency regarding aggregation. Patients with active RA present an increased EA, with values modifications associated with the activity index DAS 28-4, thus becoming an RA activity indicator.Fil: Luquita, A.. Universidad Nacional de Rosario; ArgentinaFil: Urli, L.. Universidad Nacional de Rosario; ArgentinaFil: Svetaz, M. J.. Universidad Nacional de Rosario; ArgentinaFil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Volpintesta, R.. Universidad Nacional de Rosario; ArgentinaFil: Palatnik, S.. Universidad Nacional de Rosario; ArgentinaFil: Rasia, M.. Universidad Nacional de Rosario; ArgentinaIos Press2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17122Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Volpintesta, R.; et al.; Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role; Ios Press; Clinical Hemorheology And Microcirculation; 41; 1; 10-2009; 49-561386-0291enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:47:19Zoai:ri.conicet.gov.ar:11336/17122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:47:19.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
title Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
spellingShingle Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
Luquita, A.
Rheumatoid Arthritis Activity
Erythrocyte Aggregation
Membrane Fluidity
Erythrocyte Deformability
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
title_full Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
title_fullStr Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
title_full_unstemmed Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
title_sort Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
dc.creator.none.fl_str_mv Luquita, A.
Urli, L.
Svetaz, M. J.
Gennaro, Ana Maria
Volpintesta, R.
Palatnik, S.
Rasia, M.
author Luquita, A.
author_facet Luquita, A.
Urli, L.
Svetaz, M. J.
Gennaro, Ana Maria
Volpintesta, R.
Palatnik, S.
Rasia, M.
author_role author
author2 Urli, L.
Svetaz, M. J.
Gennaro, Ana Maria
Volpintesta, R.
Palatnik, S.
Rasia, M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Rheumatoid Arthritis Activity
Erythrocyte Aggregation
Membrane Fluidity
Erythrocyte Deformability
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Rheumatoid Arthritis Activity
Erythrocyte Aggregation
Membrane Fluidity
Erythrocyte Deformability
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description ncrease in erythrocyte aggregation (EA) is pathognomonic for rheumatoid arthritis (RA), and its estimation through erythrocyte sedimentation rate (ESR) is part of DAS 28-4 activity diagnosis, with low correlation with EA and that does not discriminate the contribution of cell factors that increase aggregation. Objective: To analyse cell and plasma factors that might be involved in EA increase, to understand how RA affects blood components, thus modifying blood fluid behavior. Methodology: One hundred women presenting active RA were compared with age-matched controls (C). EA was measured by transmitted light, obtaining two parameters: 2k2n0, characterizing the aggregation process kinetics and s0/n0, estimating aggregates size. Cell factors assays: erythrocyte deformability, by filtration through nucleopore membranes, cell shape, by microscopy, and membrane fluidity by EPR. Plasma: total proteins and CRP, albumin, fibrinogen (Fb), by gravimetry, and IgG and IgM by single radial immuno-diffusion. Results: AR and C (x ± SE). 2k2n0: 31.83 ± 2.84, 23.75 ± 1.91; s0/n0: 0.92 ± 0.05, 0.87 ± 0.04. Rigidity index (RI): 14.79 ± 4.71, 6.92 ± 1.31. Morphological index: 0.28 ± 0.03, 0.30 ± 0.05, n.s. Fb (mg/dl): 382 ± 80, 299 ± 70. IgG (mg/dl): 1580 ± 219, 1296 ± 158; IgM (mg/dl) 233 ± 28, 183 ± 23; albumin (g/dl) 3.84 ± 0.44, 3.77 ± 0.51 n.s. p < 0.05 accepted. Correlations: 2k2n0 vs. Fb r = 0.66; s0/n0 vs. Fb r = 0.51; 2k2n0 vs. Igs r = 0.65; s0/n0 vs. Igs r = 0.56. 2k2n0 vs. RI r = -0.59; s0/n0 vs. RI = -0.52, p < 0.05. Conclusions: Plasma factors, Igs and Fb increased aggregation, since RI is altered, this reduces the process efficiency regarding aggregation. Patients with active RA present an increased EA, with values modifications associated with the activity index DAS 28-4, thus becoming an RA activity indicator.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17122
Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Volpintesta, R.; et al.; Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role; Ios Press; Clinical Hemorheology And Microcirculation; 41; 1; 10-2009; 49-56
1386-0291
url http://hdl.handle.net/11336/17122
identifier_str_mv Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Volpintesta, R.; et al.; Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role; Ios Press; Clinical Hemorheology And Microcirculation; 41; 1; 10-2009; 49-56
1386-0291
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Ios Press
publisher.none.fl_str_mv Ios Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195259169669120
score 15,812429

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