Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
Multiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Arg...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/86053 |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86053 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Veterinarias MIRU-VNTR MLVA Molecular typing Paratuberculosis |
| Sumario: | Multiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS<i>900</i>-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents. |
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