Structural changes induced by interactions between thyroid hormones and phospholipid membranes: a Raman Spectroscopy study

Previous reports from our laboratory have shown that thyroid hormones induce changes in the fluidity and permeability of phospholipid bilayers and modify the transmembrane dipolar organization according to their iodine content. The interactions of T2, T3, and T4 with a model membrane of dilauroylpho...

Descripción completa

Detalles Bibliográficos
Autores: Petruk, Ariel Alcides, Alvarez, Rosa Maria Susana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/21993
Acceso en línea:http://hdl.handle.net/11336/21993
Access Level:acceso abierto
Palabra clave:Thyroid Hormones
Phospholipids
Vibrational Analysis
Confocal Raman Microscopy
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
Descripción
Sumario:Previous reports from our laboratory have shown that thyroid hormones induce changes in the fluidity and permeability of phospholipid bilayers and modify the transmembrane dipolar organization according to their iodine content. The interactions of T2, T3, and T4 with a model membrane of dilauroylphosphatidylcholine in the liquid-crystalline phase were analyzed by confocal Raman spectroscopy. Insights into the nature of the hormone effects on the membrane properties, as well as the structural adaptations of the hormones in response to the lipid environment were derived from the spectral changes. The series of progressive iodine substituents on the ring-β allowed us to correlate the hormone effects according to the number and the orientation of the iodine atoms. T4 was responsible for producing the highest alteration in the hydrophobic region of the membrane.