Reduction of α-galactooligosaccharides in soyamilk by Lactobacillus fermentum CRL 722: In vitro and in vivo evaluation of fermented soyamilk

Aims: Consumption of soya-derived products has been hampered by the presence of α-galactooligosaccharides (α-GOS) because mammals lack pancreatic α-galactosidase (α-Gal) which is necessary for their hydrolysis. These sugars thus reach the large intestine causing gastrointestinal disorders in sensiti...

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Detalles Bibliográficos
Autores: Leblanc, Jean Guy Joseph, Garro, Marisa Selva, Silvestroni, Aurelio, Connes, Cristelle, Piard, Jean Christophe, Sesma, Fernando Juan Manuel, Savoy, Graciela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/57293
Acceso en línea:http://hdl.handle.net/11336/57293
Access Level:acceso abierto
Palabra clave:Α-Galactosidase
Α-Gos
Flatulence
Lactic Acid Bacteria
Soyamilk
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:Aims: Consumption of soya-derived products has been hampered by the presence of α-galactooligosaccharides (α-GOS) because mammals lack pancreatic α-galactosidase (α-Gal) which is necessary for their hydrolysis. These sugars thus reach the large intestine causing gastrointestinal disorders in sensitive individuals. The use of lactic acid bacteria (LAB) expressing α-Gal is a promising solution for the degradation of α-GOS in soyamilk. Methods and Results: The capacity of the LAB Lactobacillus fermentum CRL 722 to properly degrade α-GOS was studied in vitro using controlled fermentation conditions and in vivo using a rat model. Lactobacillus fermentum CRL 722 was able to grow on commercial soyamilk and completely eliminated stachyose and raffinose during fermentation because of its high α-Gal activity. Rats fed soyamilk fermented by this LAB had smaller caecums compared with rats fed unfermented soyamilk. Conclusions: Soyamilk fermentation by Lact. fermentum CRL 722 results in the reduction of α-GOS concentrations in soyamilk, thus eliminating possible undesirable physiological effects normally associated with its consumption. Significance and Impact of the Study: Fermentation with Lact. fermentum CRL 722 could prevent gastrointestinal disorders in sensitive individuals normally associated with the consumption of soya-based products. This LAB could thus be used in the elaboration of novel fermented vegetable products which better suit the digestive capacities of consumers.