Involvement of SARA in axon and dendrite growth

SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis in cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the role of SARA during neuronal development and tested whether it functions as a regulator of endocy...

Descripción completa

Detalles Bibliográficos
Autores: Arias, Cristina Isabel, Siri, Sebastian Omar, Conde, Cecilia Beatriz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/69401
Acceso en línea:http://hdl.handle.net/11336/69401
Access Level:acceso abierto
Palabra clave:AXON
DENDRITE
POLARITY
ENDOSOMES
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_02f4d4cd36a65fe8a8d7e1f8675bcf2e
oai_identifier_str oai:ri.conicet.gov.ar:11336/69401
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Involvement of SARA in axon and dendrite growthArias, Cristina IsabelSiri, Sebastian OmarConde, Cecilia BeatrizAXONDENDRITEPOLARITYENDOSOMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis in cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the role of SARA during neuronal development and tested whether it functions as a regulator of endocytic trafficking of selected axonal and membrane proteins. Suppression of SARA perturbs the appearance of juxtanuclear endocytic recycling compartments and the neurons show long axons with large growth cones. Furthermore, surface distribution of the cell adhesion molecule L1 in axons and the fusion of vesicles containing transferring receptor (TfR) in dendrites were increased in neurons where SARA was silenced. Conversely, SARA overexpression generated large early endosomes and reduced neurite outgrowth. Taken together, our findings suggest a significant contribution of SARA to key aspects of neuronal development, including neurite formation. Copyright:Fil: Arias, Cristina Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Siri, Sebastian Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Conde, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaPublic Library of Science2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69401Arias, Cristina Isabel; Siri, Sebastian Omar; Conde, Cecilia Beatriz; Involvement of SARA in axon and dendrite growth; Public Library of Science; Plos One; 10; 9; 9-2015; 1-191932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0138792info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138792info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:16:04Zoai:ri.conicet.gov.ar:11336/69401instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:16:05.239CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of SARA in axon and dendrite growth
title Involvement of SARA in axon and dendrite growth
spellingShingle Involvement of SARA in axon and dendrite growth
Arias, Cristina Isabel
AXON
DENDRITE
POLARITY
ENDOSOMES
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Involvement of SARA in axon and dendrite growth
title_full Involvement of SARA in axon and dendrite growth
title_fullStr Involvement of SARA in axon and dendrite growth
title_full_unstemmed Involvement of SARA in axon and dendrite growth
title_sort Involvement of SARA in axon and dendrite growth
dc.creator.none.fl_str_mv Arias, Cristina Isabel
Siri, Sebastian Omar
Conde, Cecilia Beatriz
author Arias, Cristina Isabel
author_facet Arias, Cristina Isabel
Siri, Sebastian Omar
Conde, Cecilia Beatriz
author_role author
author2 Siri, Sebastian Omar
Conde, Cecilia Beatriz
author2_role author
author
dc.subject.none.fl_str_mv AXON
DENDRITE
POLARITY
ENDOSOMES
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic AXON
DENDRITE
POLARITY
ENDOSOMES
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis in cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the role of SARA during neuronal development and tested whether it functions as a regulator of endocytic trafficking of selected axonal and membrane proteins. Suppression of SARA perturbs the appearance of juxtanuclear endocytic recycling compartments and the neurons show long axons with large growth cones. Furthermore, surface distribution of the cell adhesion molecule L1 in axons and the fusion of vesicles containing transferring receptor (TfR) in dendrites were increased in neurons where SARA was silenced. Conversely, SARA overexpression generated large early endosomes and reduced neurite outgrowth. Taken together, our findings suggest a significant contribution of SARA to key aspects of neuronal development, including neurite formation. Copyright:
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69401
Arias, Cristina Isabel; Siri, Sebastian Omar; Conde, Cecilia Beatriz; Involvement of SARA in axon and dendrite growth; Public Library of Science; Plos One; 10; 9; 9-2015; 1-19
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69401
identifier_str_mv Arias, Cristina Isabel; Siri, Sebastian Omar; Conde, Cecilia Beatriz; Involvement of SARA in axon and dendrite growth; Public Library of Science; Plos One; 10; 9; 9-2015; 1-19
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0138792
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138792
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196138060906496
score 15.812429

Similares en LA Referencia