Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography

The quantitative determination of boldine alkaloid in boldo leaf extracts by employing cyclic voltammetry, at a liquid/liquid interface as well as the validation of this methodology against the reference method, high performance liquid chromatography (HPLC), are reported in the present paper. The vo...

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Authors: Cámara, Candelaria Inés, Bornancini, Carlos Alberto, Cabrera, Jose Luis, Ortega, María Gabriela, Yudi, Lidia Mabel
Format: article
Status:Published version
Publication Date:2010
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/69832
Online Access:http://hdl.handle.net/11336/69832
Access Level:Open access
Keyword:Acid Dissociation Constant
Boldine Alkaloids
Electrochemistry at Liquid/Liquid Interfaces
Quantification of Complex Matrixes
Validation of Electrochemical Method
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/69832
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
title Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
spellingShingle Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
Cámara, Candelaria Inés
Acid Dissociation Constant
Boldine Alkaloids
Electrochemistry at Liquid/Liquid Interfaces
Quantification of Complex Matrixes
Validation of Electrochemical Method
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
title_full Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
title_fullStr Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
title_full_unstemmed Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
title_sort Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
dc.creator.none.fl_str_mv Cámara, Candelaria Inés
Bornancini, Carlos Alberto
Cabrera, Jose Luis
Ortega, María Gabriela
Yudi, Lidia Mabel
author Cámara, Candelaria Inés
author_facet Cámara, Candelaria Inés
Bornancini, Carlos Alberto
Cabrera, Jose Luis
Ortega, María Gabriela
Yudi, Lidia Mabel
author_role author
author2 Bornancini, Carlos Alberto
Cabrera, Jose Luis
Ortega, María Gabriela
Yudi, Lidia Mabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Acid Dissociation Constant
Boldine Alkaloids
Electrochemistry at Liquid/Liquid Interfaces
Quantification of Complex Matrixes
Validation of Electrochemical Method
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic Acid Dissociation Constant
Boldine Alkaloids
Electrochemistry at Liquid/Liquid Interfaces
Quantification of Complex Matrixes
Validation of Electrochemical Method
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description The quantitative determination of boldine alkaloid in boldo leaf extracts by employing cyclic voltammetry, at a liquid/liquid interface as well as the validation of this methodology against the reference method, high performance liquid chromatography (HPLC), are reported in the present paper. The voltammetric analysis was performed successfully and economically using two kinds of liquid/liquid interfaces: water/1,2-dicholoroethane and water/PVC (polyvinyl chloride)-gelled 1,2-dichloroethane. Linear calibration curves in the concentration range of 1.04 × 10-5 mol L-1 to 5.19 × 10-4 mol L-1 were obtained with a detection limit equal to (6.1 ± 0.7) × 10-5 mol L-1 and the quantitative determination of this alkaloid, in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was found to be equal to the values obtained using the standard HPLC method. The validation analysis of this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed is economical and selective, involves simple equipment and can be applied for the quantitative determination of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation. Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value of pKa w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69832
Cámara, Candelaria Inés; Bornancini, Carlos Alberto; Cabrera, Jose Luis; Ortega, María Gabriela; Yudi, Lidia Mabel; Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography; Elsevier Science; Talanta; 83; 2; 12-2010; 623-630
0039-9140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69832
identifier_str_mv Cámara, Candelaria Inés; Bornancini, Carlos Alberto; Cabrera, Jose Luis; Ortega, María Gabriela; Yudi, Lidia Mabel; Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography; Elsevier Science; Talanta; 83; 2; 12-2010; 623-630
0039-9140
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S003991401000785X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2010.10.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatographyCámara, Candelaria InésBornancini, Carlos AlbertoCabrera, Jose LuisOrtega, María GabrielaYudi, Lidia MabelAcid Dissociation ConstantBoldine AlkaloidsElectrochemistry at Liquid/Liquid InterfacesQuantification of Complex MatrixesValidation of Electrochemical Methodhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The quantitative determination of boldine alkaloid in boldo leaf extracts by employing cyclic voltammetry, at a liquid/liquid interface as well as the validation of this methodology against the reference method, high performance liquid chromatography (HPLC), are reported in the present paper. The voltammetric analysis was performed successfully and economically using two kinds of liquid/liquid interfaces: water/1,2-dicholoroethane and water/PVC (polyvinyl chloride)-gelled 1,2-dichloroethane. Linear calibration curves in the concentration range of 1.04 × 10-5 mol L-1 to 5.19 × 10-4 mol L-1 were obtained with a detection limit equal to (6.1 ± 0.7) × 10-5 mol L-1 and the quantitative determination of this alkaloid, in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was found to be equal to the values obtained using the standard HPLC method. The validation analysis of this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed is economical and selective, involves simple equipment and can be applied for the quantitative determination of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation. Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value of pKa w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.Fil: Cámara, Candelaria Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bornancini, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Yudi, Lidia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69832Cámara, Candelaria Inés; Bornancini, Carlos Alberto; Cabrera, Jose Luis; Ortega, María Gabriela; Yudi, Lidia Mabel; Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography; Elsevier Science; Talanta; 83; 2; 12-2010; 623-6300039-9140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S003991401000785Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2010.10.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:02:27Zoai:ri.conicet.gov.ar:11336/69832instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:02:27.653CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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