Microstructure characterization through mechanical, electrokinetic and spectroscopic methods of polyampholyte gelatin hydrogels crosslinked with poly(vinyl alcohol)

The microstructure of polyampholyte gelatin hydrogels crosslinked covalently with fully hydrolyzed poly(vinyl alcohol) is characterized. The experimental methodology involves simple mechanical extension, spectroscopic and thermal methods (FTIR, XRD, DSC, TGA) and equilibrium swelling test to quantif...

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Detalles Bibliográficos
Autores: Porcaro, Andrea, Ottone, Mariel Lorena, Deiber, Julio Alcides
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/8870
Acceso en línea:http://hdl.handle.net/11336/8870
Access Level:acceso abierto
Palabra clave:Gelatin-Poly(Vinyl Alcohol) Hydrogel Characterization
Hydrogel Mechanical Properties
Hydrogel Electrokinetic Properties
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
Descripción
Sumario:The microstructure of polyampholyte gelatin hydrogels crosslinked covalently with fully hydrolyzed poly(vinyl alcohol) is characterized. The experimental methodology involves simple mechanical extension, spectroscopic and thermal methods (FTIR, XRD, DSC, TGA) and equilibrium swelling test to quantify relevant microstructural parameters and electrokinetic properties of this polyampholyte-polypeptide hydrogel, for a well defined range of crosslinker to gelatin mass ratios. These polyampholyte crosslinked matrices having different positive effective electrical charge are studied within an experimental and theoretical framework of their elastic and swelling properties. Estimations of average mesh size and toughness of the swollen hydrogels are provided. The feasibility of polyion complexation between positive crosslinked gelatin chains and negative bioactive macromolecules to be delivered through hydrogel biodegradation is analyzed. Several additional hydrogel properties are estimated like shear modulus, average molecular mass of network strands, swelling ratio, FloryeHuggins interaction parameter, hydrogel electrostatic zeta-potential and average effective charge number per chain before and after the crosslinking process to quantify gelatin chain cationization.