Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic

Rod-shaped fission yeast grows through cell wall expansion at poles and septum, synthesized by essential glucan synthases. Bgs1 synthesizes the linear β(1,3)glucan of primary septum at cytokinesis. Linear β(1,3)glucan is also present in the wall poles, suggesting additional Bgs1 roles in growth pola...

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Detalles Bibliográficos
Autores: Ramos, Mariona, Martín-García, Rebeca, Curto, María Ángeles, Gómez-Delgado, Laura, Moreno, M. Belén, Sato, Mamiko, Portales, Elvira, Osumi, Masako, Rincón, Sergio A., Pérez González, Pilar, Ribas, Juan Carlos, Cortés, Juan Carlos G.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/378958
Acceso en línea:http://hdl.handle.net/10261/378958
https://api.elsevier.com/content/abstract/scopus_id/85199055530
Access Level:acceso abierto
Palabra clave:Functional aspects of cell biology
Mycology
Organizational aspects of cell biology
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spelling Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane trafficRamos, MarionaMartín-García, RebecaCurto, María ÁngelesGómez-Delgado, LauraMoreno, M. BelénSato, MamikoPortales, ElviraOsumi, MasakoRincón, Sergio A.Pérez González, PilarRibas, Juan CarlosCortés, Juan Carlos G.Functional aspects of cell biologyMycologyOrganizational aspects of cell biologyRod-shaped fission yeast grows through cell wall expansion at poles and septum, synthesized by essential glucan synthases. Bgs1 synthesizes the linear β(1,3)glucan of primary septum at cytokinesis. Linear β(1,3)glucan is also present in the wall poles, suggesting additional Bgs1 roles in growth polarity. Our study reveals an essential collaboration between Bgs1 and Tea1-Tea4, but not other polarity factors, in controlling growth polarity. Simultaneous absence of Bgs1 function and Tea1-Tea4 causes complete loss of growth polarity, spread of other glucan synthases, and spherical cell formation, indicating this defect is specifically due to linear β(1,3)glucan absence. Furthermore, linear β(1,3)glucan absence induces actin patches delocalization and sterols spread, which are ultimately responsible for the growth polarity loss without Tea1-Tea4. This suggests strong similarities in Bgs1 functions controlling actin structures during cytokinesis and polarized growth. Collectively, our findings unveil that cell wall β(1,3)glucan regulates polarized growth, like the equivalent extracellular matrix in neuronal cells.This work was supported by grants: PGC2018-098924-B-100, PID2021-124971NB-I00, PID2021-127459NB-I00, and CNS2022-135153 (Ministerio de Ciencia, Innovación y Universidades, MICIU/AEI/10.13039/501100011033, Spain and ERDF A way of making Europe, EU); and CSI150P20, “Escalera de Excelencia” CLU-2017-03, and “Internacionalización” CL-EI-2021-08 (Junta de Castilla y León, Spain and ERDF, EU).Peer reviewedCell PressAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación (España)European CommissionJunta de Castilla y LeónRibas, Juan Carlos [0000-0001-6430-0895]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/378958https://api.elsevier.com/content/abstract/scopus_id/85199055530reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-098924-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-127459NB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124971NB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.isci.2024.110477https://doi.org/10.1016/j.isci.2024.110477Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3789582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
title Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
spellingShingle Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
Ramos, Mariona
Functional aspects of cell biology
Mycology
Organizational aspects of cell biology
title_short Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
title_full Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
title_fullStr Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
title_full_unstemmed Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
title_sort Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
dc.creator.none.fl_str_mv Ramos, Mariona
Martín-García, Rebeca
Curto, María Ángeles
Gómez-Delgado, Laura
Moreno, M. Belén
Sato, Mamiko
Portales, Elvira
Osumi, Masako
Rincón, Sergio A.
Pérez González, Pilar
Ribas, Juan Carlos
Cortés, Juan Carlos G.
author Ramos, Mariona
author_facet Ramos, Mariona
Martín-García, Rebeca
Curto, María Ángeles
Gómez-Delgado, Laura
Moreno, M. Belén
Sato, Mamiko
Portales, Elvira
Osumi, Masako
Rincón, Sergio A.
Pérez González, Pilar
Ribas, Juan Carlos
Cortés, Juan Carlos G.
author_role author
author2 Martín-García, Rebeca
Curto, María Ángeles
Gómez-Delgado, Laura
Moreno, M. Belén
Sato, Mamiko
Portales, Elvira
Osumi, Masako
Rincón, Sergio A.
Pérez González, Pilar
Ribas, Juan Carlos
Cortés, Juan Carlos G.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Junta de Castilla y León
Ribas, Juan Carlos [0000-0001-6430-0895]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Functional aspects of cell biology
Mycology
Organizational aspects of cell biology
topic Functional aspects of cell biology
Mycology
Organizational aspects of cell biology
description Rod-shaped fission yeast grows through cell wall expansion at poles and septum, synthesized by essential glucan synthases. Bgs1 synthesizes the linear β(1,3)glucan of primary septum at cytokinesis. Linear β(1,3)glucan is also present in the wall poles, suggesting additional Bgs1 roles in growth polarity. Our study reveals an essential collaboration between Bgs1 and Tea1-Tea4, but not other polarity factors, in controlling growth polarity. Simultaneous absence of Bgs1 function and Tea1-Tea4 causes complete loss of growth polarity, spread of other glucan synthases, and spherical cell formation, indicating this defect is specifically due to linear β(1,3)glucan absence. Furthermore, linear β(1,3)glucan absence induces actin patches delocalization and sterols spread, which are ultimately responsible for the growth polarity loss without Tea1-Tea4. This suggests strong similarities in Bgs1 functions controlling actin structures during cytokinesis and polarized growth. Collectively, our findings unveil that cell wall β(1,3)glucan regulates polarized growth, like the equivalent extracellular matrix in neuronal cells.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/378958
https://api.elsevier.com/content/abstract/scopus_id/85199055530
url http://hdl.handle.net/10261/378958
https://api.elsevier.com/content/abstract/scopus_id/85199055530
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-098924-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-127459NB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124971NB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.isci.2024.110477
https://doi.org/10.1016/j.isci.2024.110477
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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