Generation of the Brucella melitensis ORFeome version 1.1.

The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecula...

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Autores: Dricot, A. (Amélie)|||/items/cb0cea57-2beb-4f62-9bea-22928061e377, Rual, J. J. (Jean François)|||/items/d40d67d0-fc99-42b3-90eb-9d864a4cbafd, Lamesch, P. (Philippe)|||/items/ba8c4e87-3a83-4ee6-ad3d-ecc250814764, Bertin, N. (Nicolas)|||/items/9a0674a0-d0d0-45ba-8e3a-5a93cd3ddb63, Dupuy, D. (Denis)|||/items/08cd7423-d0b1-4481-a83a-4c39c879d17f, Hao, T. (Tong)|||/items/9becb58b-15bf-48b9-944e-f5da20c957bb, Lambert, C. (Christophe)|||/items/0f33bb40-05fc-4faa-8b8e-f178e9777dd6, Hallez, R. (Régis)|||/items/8a97960e-c4f0-4c92-b743-261c09f65469, Delroisse, J.M. (Jean Marc)|||/items/ac7760ec-ae10-4694-8a3f-07c1e6fed0c0, Vandenhaute, J. (Jean)|||/items/2f01accc-7109-4b2c-9057-0a4f2362fefd, López-Goñi, I. (Ignacio)|||/items/228678b8-8965-4c7c-aa6f-493277285d88, Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b, Garcia-Lobo, J.M. (Juan María)|||/items/a0fb156a-53ce-459b-b499-f2aef78a980c, Sangari, F.J. (Félix Javier)|||/items/30fdfc19-f831-4cf5-b839-c7b6aff385a6, MacMillan, A.P. (Alastair P.)|||/items/75df6703-1019-4431-a1a3-857b3be6525e, Cutler, S.J. (Sally J.)|||/items/29fedef7-f0ad-4417-a53c-7239a3e12496, Whatmore, A.M. (Adrian M.)|||/items/f96f8d99-c90e-40b8-86a4-7f0a460c475d, Bozak, S. (Stephanie)|||/items/6aa60cde-4dac-4ac8-831c-9d52fdad7871, Sequerra, R. (Reynaldo)|||/items/8a070a66-a09b-4be1-83ea-141987788597, Doucettte-Stamm, L. (Lynn)|||/items/c648368c-7b27-496e-a922-66b7a2873d58, Vidal, M. (Marc)|||/items/1265c8a7-110d-44ce-bb22-93ca01877a2a, Hill, D.E. (David E.)|||/items/6c35f648-f361-4f19-a1a0-2a9f4ca1894d, Letesson, J.J. (Jean Jacques)|||/items/310147b5-d631-4025-ad82-d3e696cc9a4c, Bolle, X. (Xavier) de|||/items/4e775989-350e-41ce-bbfc-4f4a0eee72ac
Tipo de recurso: artículo
Fecha de publicación:2004
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/29445
Acceso en línea:https://hdl.handle.net/10171/29445
Access Level:acceso abierto
Palabra clave:Bacterial proteins genetics
Brucella melitensis genetics
Genome, bacterial
Open reading frames physiology
Descripción
Sumario:The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of 3091 Gateway Entry clones, each containing a defined ORF. This first version of the Brucella ORFeome (v1.1) provides the coding sequences in a user-friendly format amenable to high-throughput functional genomic and proteomic experiments, as the ORFs are conveniently transferable from the Entry clones to various Expression vectors by recombinational cloning. The cloning of the Brucella ORFeome v1.1 should help to provide a better understanding of the molecular mechanisms of virulence, including the identification of bacterial protein-protein interactions, but also interactions between bacterial effectors and their host's targets.

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