Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway

Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indica...

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Bibliographic Details
Authors: Sarri Plans, Elisabet, Martínez Rodríguez, Susana Eva, Jiménez, Nuria, Claro Izaguirre, Enrique, Mérida, Isabel, Burger, Koert N. J., Egea Guri, Gustavo, Fernández Ulibarri, Inés, Vilella, Montserrat, Lázaro Diégez, Francisco
Format: article
Status:Published version
Publication Date:2007
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/24655
Online Access:https://hdl.handle.net/2445/24655
http://dx.doi.org/10.1091/mbc.E07-04-0334
Access Level:Open access
Keyword:Aparell de Golgi
Reticle endoplasmàtic
Proteïnes
Citologia
Transport biològic
Golgi apparatus
Endoplasmic reticulum
Proteins
Cytology
Biological transport
Description
Summary:Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation.

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