Development of monoclonal antibody-based competitive immunoassays for the detection of picoxystrobin in cereal and oilseed flours
Picoxystrobin is a new generation fungicide primarily developed to be used in cereal crops. In the present study a novel collection of specific monoclonal antibodies has been produced using different immunizing haptens based on a carboxy-functionalized identical spacer arm attached to alternative po...
| Autores: | , , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/112223 |
| Acesso em linha: | http://hdl.handle.net/10261/112223 |
| Access Level: | acceso abierto |
| Palavra-chave: | ELISA Hapten Food safety Strobilurins Pesticide residues Spacer arm |
| Resumo: | Picoxystrobin is a new generation fungicide primarily developed to be used in cereal crops. In the present study a novel collection of specific monoclonal antibodies has been produced using different immunizing haptens based on a carboxy-functionalized identical spacer arm attached to alternative positions of the target pesticide molecule. Two competitive enzyme-linked immunosorbent assays have been developed employing hapten heterology, one using the antibody-coated direct format and the other in the conjugate-coated indirect format. Both immunoassays have been characterized in terms of selectivity, solvent tolerance, and buffer conditions, affording similar limits of detection at or below 0.1 μg/L. Finally, the optimized assays were applied to the analysis of picoxystrobin in wheat, corn, oat, barley, and soybean flours. Average recovery values from spiked samples were between 84 and 115%. |
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