B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines

Synthetic peptides incorporating protective B- and T-cell epitopes are candidates for new safer foot-and-mouth disease (FMD) vaccines. We have reported that dendrimeric peptides including four copies of a B-cell epitope (VP1 136 to 154) linked to a T-cell epitope (3A 21 to 35) of FMD virus (FMDV) el...

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Detalles Bibliográficos
Autores: Blanco, Esther, Cubillos, Carolina, Moreno, Noelia, Bárcena, Juan, Torre, Beatriz G. de la, Andreu Martínez, David, Sobrino, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/25214
Acceso en línea:http://hdl.handle.net/10230/25214
http://dx.doi.org/10.1155/2013/475960
Access Level:acceso abierto
Palabra clave:Immunoglobulines
Pèptids
Cèl·lules T
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spelling B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccinesBlanco, EstherCubillos, CarolinaMoreno, NoeliaBárcena, JuanTorre, Beatriz G. de laAndreu Martínez, DavidSobrino, FranciscoImmunoglobulinesPèptidsCèl·lules TSynthetic peptides incorporating protective B- and T-cell epitopes are candidates for new safer foot-and-mouth disease (FMD) vaccines. We have reported that dendrimeric peptides including four copies of a B-cell epitope (VP1 136 to 154) linked to a T-cell epitope (3A 21 to 35) of FMD virus (FMDV) elicit potent B- and T-cell specific responses and confer protection to viral challenge, while juxtaposition of these epitopes in a linear peptide induces less efficient responses. To assess the relevance of B-cell epitope multivalency, dendrimers bearing two (B2T) or four (B4T) copies of the B-cell epitope from type O FMDV (a widespread circulating serotype) were tested in CD1 mice and showed that multivalency is advantageous over simple B-T-epitope juxtaposition, resulting in efficient induction of neutralizing antibodies and optimal release of IFN γ . Interestingly, the bivalent B2T construction elicited similar or even better B- and T-cell specific responses than tetravalent B4T. In addition, the presence of the T-cell epitope and its orientation were shown to be critical for the immunogenicity of the linear juxtaposed monovalent peptides analyzed in parallel. Taken together, our results provide useful insights for a more accurate design of FMD subunit vaccines.Research at CBMSO was funded by Spanish MINECO (BIO2011-24351), and by an institutional grant from Fundación Ramón Areces. Research at Universitat Pompeu Fabra was funded by the Spanish MINECO (SAF2011-24899) and by Generalitat de Catalunya (SGR2009-00492). Research at INIA was funded by the European Community’s Seventh Framework Programme (FP7, 2007-2013), Research Infrastructures action, under the grant agreement no. FP7-228394 (NADIR) and Spanish MINECO (AGL2010-22200-C02-02 and CC07-062). Noelia Moreno is a predoctoral (FPI) fellow from the Spanish MEC.Taylor & Francis Health (Routledge)201520152013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25214http://dx.doi.org/10.1155/2013/475960reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésClinical & developmental immunology. 2013;2013:475960© 2013 Esther Blanco et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/252142026-06-12T07:21:37Z
dc.title.none.fl_str_mv B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
title B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
spellingShingle B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
Blanco, Esther
Immunoglobulines
Pèptids
Cèl·lules T
title_short B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
title_full B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
title_fullStr B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
title_full_unstemmed B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
title_sort B epitope multiplicity and B/T epitope orientation influence immunogenicity of foot-and-mouth disease peptide vaccines
dc.creator.none.fl_str_mv Blanco, Esther
Cubillos, Carolina
Moreno, Noelia
Bárcena, Juan
Torre, Beatriz G. de la
Andreu Martínez, David
Sobrino, Francisco
author Blanco, Esther
author_facet Blanco, Esther
Cubillos, Carolina
Moreno, Noelia
Bárcena, Juan
Torre, Beatriz G. de la
Andreu Martínez, David
Sobrino, Francisco
author_role author
author2 Cubillos, Carolina
Moreno, Noelia
Bárcena, Juan
Torre, Beatriz G. de la
Andreu Martínez, David
Sobrino, Francisco
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunoglobulines
Pèptids
Cèl·lules T
topic Immunoglobulines
Pèptids
Cèl·lules T
description Synthetic peptides incorporating protective B- and T-cell epitopes are candidates for new safer foot-and-mouth disease (FMD) vaccines. We have reported that dendrimeric peptides including four copies of a B-cell epitope (VP1 136 to 154) linked to a T-cell epitope (3A 21 to 35) of FMD virus (FMDV) elicit potent B- and T-cell specific responses and confer protection to viral challenge, while juxtaposition of these epitopes in a linear peptide induces less efficient responses. To assess the relevance of B-cell epitope multivalency, dendrimers bearing two (B2T) or four (B4T) copies of the B-cell epitope from type O FMDV (a widespread circulating serotype) were tested in CD1 mice and showed that multivalency is advantageous over simple B-T-epitope juxtaposition, resulting in efficient induction of neutralizing antibodies and optimal release of IFN γ . Interestingly, the bivalent B2T construction elicited similar or even better B- and T-cell specific responses than tetravalent B4T. In addition, the presence of the T-cell epitope and its orientation were shown to be critical for the immunogenicity of the linear juxtaposed monovalent peptides analyzed in parallel. Taken together, our results provide useful insights for a more accurate design of FMD subunit vaccines.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25214
http://dx.doi.org/10.1155/2013/475960
url http://hdl.handle.net/10230/25214
http://dx.doi.org/10.1155/2013/475960
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Clinical & developmental immunology. 2013;2013:475960
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Health (Routledge)
publisher.none.fl_str_mv Taylor & Francis Health (Routledge)
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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