X chromosome status : a gatekeeper of germ cells meiotic entry
X chromosome reactivation in female mouse germ cells is essential for the transmission of one active X chromosome to the progeny. However, despite its key role in development, the mechanistic details and kinetics still remain elusive, as previous studies were restricted by a scarcity of cells in viv...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/671536 |
| Acceso en línea: | http://hdl.handle.net/10803/671536 |
| Access Level: | acceso abierto |
| Palabra clave: | Germline X chromosome reactivation Meiosis Epigenetic reprogramming Células madre Línea germinal Reactivación del cromosoma X Reprogramación epigenética 576 |
| Sumario: | X chromosome reactivation in female mouse germ cells is essential for the transmission of one active X chromosome to the progeny. However, despite its key role in development, the mechanistic details and kinetics still remain elusive, as previous studies were restricted by a scarcity of cells in vivo and a lack of adequate in vitro systems. Here, I present the characterization of X-chromosome dynamics during germ cell formation, which was possible thanks to the development of a tailor-made in vitro system which allows the accurate profiling of X-chromosome activity. We recapitulate X-inactivation starting in epiblast-like cells (EpiLCs), progressing in primordial germ cell-like cells (PGCLCs), followed by X-reactivation in germ cells upon meiotic entry. We show that PGCLCs undergoing X-inactivation can enter meiosis more efficiently, while PGCLCs bypassing X-inactivation, with the consequent lack of X-reactivation, show a reduced meiotic potential. We conclude that tracing the X chromosome status during germ cell formation facilitates the dissection of the relationship between X chromosome dynamics and proper germline fate acquisition |
|---|