Structural analysis of macromolecular nanomachines by 3d electron microscopy: Managing flexibility and heterogenety in some define cases

The term macromolecular nanomachines refers to those complexes that correspond to the functional machinery in the context of the living cell,, from DNA repair or replication, to protein degradation or cell división, to name just a few functions. Normally, they are formed by different types of protei...

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Detalles Bibliográficos
Autor: Álvarez Cabrera, Ana Lucía
Tipo de recurso: tesis doctoral
Fecha de publicación:2015
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/674113
Acceso en línea:http://hdl.handle.net/10486/674113
Access Level:acceso abierto
Palabra clave:Microscopía electrónica - Tesis doctorales
Nanotecnología - Tesis doctorales
Biología y Biomedicina / Biología
Descripción
Sumario:The term macromolecular nanomachines refers to those complexes that correspond to the functional machinery in the context of the living cell,, from DNA repair or replication, to protein degradation or cell división, to name just a few functions. Normally, they are formed by different types of proteins and may also include nucleic acids. The estructure of macromolecular nanomachines and their structural changes is a very important process with a vital role in functional implications. Their study is many times accomplished by three-dimensional elecron microscopy, since they are too large for Nuclear Magnetic Resonance and too flexible for X-ray Diffraction. In these termes, Electron microscopy has provided important structdural information about protein complexes essential for describing their functional organization. Understanding how function occurs can only be inferred after integrating many different types of data