Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter

Electronic Supplementary Information (ESI) available: Supplementary figures and tables and copies of the 1H and 13C NMR (1D and 2D) spectra of the new compounds.

Detalhes bibliográficos
Autores: Blázquez-Moraleja, Alberto, Sáenz-de-Santa María, Inés, Chiara, María-Dolores, Álvarez- Fernández, Delia, García-Moreno, I., Prieto Montero, Ruth, Martínez-Martínez, Virginia, López-Arbeloa, Íñigo, Chiara, José Luis
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/196537
Acesso em linha:http://hdl.handle.net/10261/196537
Access Level:Acceso aberto
Palavra-chave:Fluorescent dyes
BODIPY dyes
Cell microscopy
Mitochondria
Biomimetics
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oai_identifier_str oai:digital.csic.es:10261/196537
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
title Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
spellingShingle Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
Blázquez-Moraleja, Alberto
Fluorescent dyes
BODIPY dyes
Cell microscopy
Mitochondria
Biomimetics
title_short Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
title_full Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
title_fullStr Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
title_full_unstemmed Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
title_sort Shedding Light on the Mitochondrial Matrix Through a Functional Membrane Transporter
dc.creator.none.fl_str_mv Blázquez-Moraleja, Alberto
Sáenz-de-Santa María, Inés
Chiara, María-Dolores
Álvarez- Fernández, Delia
García-Moreno, I.
Prieto Montero, Ruth
Martínez-Martínez, Virginia
López-Arbeloa, Íñigo
Chiara, José Luis
author Blázquez-Moraleja, Alberto
author_facet Blázquez-Moraleja, Alberto
Sáenz-de-Santa María, Inés
Chiara, María-Dolores
Álvarez- Fernández, Delia
García-Moreno, I.
Prieto Montero, Ruth
Martínez-Martínez, Virginia
López-Arbeloa, Íñigo
Chiara, José Luis
author_role author
author2 Sáenz-de-Santa María, Inés
Chiara, María-Dolores
Álvarez- Fernández, Delia
García-Moreno, I.
Prieto Montero, Ruth
Martínez-Martínez, Virginia
López-Arbeloa, Íñigo
Chiara, José Luis
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Eusko Jaurlaritza
Instituto de Salud Carlos III
Blázquez, Alberto [0000-0003-1375-930X]
Sáenz-deChiara-Santa María, Inés [0000-0002-1868-3001]
Chiara, María D. [0000-0002-1112-1583]
García-Moreno, Inmaculada [0000-0002-8682-3715]
Prieto-Montero, Ruth [0000-0001-6372-563X]
Martínez-Martínez, Virginia [0000-0001-7551-3714]
López-Arbeloa, Íñigo [0000-0002-8440-6600]
Chiara, José Luis [0000-0002-8153-1852]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fluorescent dyes
BODIPY dyes
Cell microscopy
Mitochondria
Biomimetics
topic Fluorescent dyes
BODIPY dyes
Cell microscopy
Mitochondria
Biomimetics
description Electronic Supplementary Information (ESI) available: Supplementary figures and tables and copies of the 1H and 13C NMR (1D and 2D) spectra of the new compounds.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/196537
url http://hdl.handle.net/10261/196537
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/MAT2017-83856-C3-1-P
MAT2017-83856-C3-3-P/AEI/10.13039/501100011033
MAT2017-83856-C3-1-P/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/MAT2017-83856-C3-3-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2015-68837-REDT
http://dx.doi.org/10.1039/C9SC04852A
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Shedding Light on the Mitochondrial Matrix Through a Functional Membrane TransporterBlázquez-Moraleja, AlbertoSáenz-de-Santa María, InésChiara, María-DoloresÁlvarez- Fernández, DeliaGarcía-Moreno, I.Prieto Montero, RuthMartínez-Martínez, VirginiaLópez-Arbeloa, ÍñigoChiara, José LuisFluorescent dyesBODIPY dyesCell microscopyMitochondriaBiomimeticsElectronic Supplementary Information (ESI) available: Supplementary figures and tables and copies of the 1H and 13C NMR (1D and 2D) spectra of the new compounds.The first fluorescent probes that are actively channeled into the mitochondrial matrix by a specific mitochondrial membrane transporter in living cells have been developed. The new functional probes (BCT) have a minimalist structural design based on the highly efficient and photostable BODIPY chromophore and carnitine as a biotargeting element. Both units are orthogonally bonded through the common boron atom, thus avoiding the use of complex polyatomic connectors. In contrast to known mitochondria-specific dyes, BCTs selectively label these organelles regardless of their transmembrane potential and in an enantioselective way. The obtained experimental evidence supports carnitine-acylcarnitine translocase (CACT) as the key transporter protein for BCTs, which behave therefore as acylcarnitine biomimetics. This simple structural design can be readily extended to other structurally diverse starting FBODIPYs to obtain BCTs with varied emission wavelengths along the visible and NIR spectral regions and with multifunctional capabilities. BCTs are the first fluorescent derivatives of carnitine to be used in cell microscopy and stand as promising research tools to explore the role of the carnitine shuttle system in cancer and metabolic diseases. Extension of this approach to other small-molecule mitochondrial transporters is envisaged.We gratefully acknowledge the Spanish Ministerio de Ciencia, Innovación y Universidades (MCIU), Agencia Estatal de Investigación (AEI), and European Regional Development Fund (ERDF) (projects MAT2017-83856-C3-1-P, MAT2017-83856-C3-3-P, and MAT2015-68837-REDT), Gobierno Vasco (IT912-16), and the Instituto de Salud Carlos III-Fondo de Investigación Sanitaria (CIBERONC and PI17/01901) for financial support. A. B.-M. and R. P. M. thank MCIU, AEI and ERDF, and UPV-EHU for a FPI predoctoral contract and predoctoral fellowship, respectivelyPeer reviewedRoyal Society of Chemistry (UK)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionEusko JaurlaritzaInstituto de Salud Carlos IIIBlázquez, Alberto [0000-0003-1375-930X]Sáenz-deChiara-Santa María, Inés [0000-0002-1868-3001]Chiara, María D. [0000-0002-1112-1583]García-Moreno, Inmaculada [0000-0002-8682-3715]Prieto-Montero, Ruth [0000-0001-6372-563X]Martínez-Martínez, Virginia [0000-0001-7551-3714]López-Arbeloa, Íñigo [0000-0002-8440-6600]Chiara, José Luis [0000-0002-8153-1852]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/196537reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/MAT2017-83856-C3-1-PMAT2017-83856-C3-3-P/AEI/10.13039/501100011033MAT2017-83856-C3-1-P/AEI/10.13039/501100011033info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/MAT2017-83856-C3-3-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2015-68837-REDThttp://dx.doi.org/10.1039/C9SC04852ASíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1965372026-05-22T06:33:51Z
score 15.81155

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