Ulmus minor response to Dutch elm disease: de novo transcriptome assembly and annotation
Dutch elm disease (DED), caused by Ophiostoma novo-ulmi (ONU), has devastated elm populations across Europe and North America since the 20th century. In this work, a de novo transcriptome assembly of Ulmus minor in response to ONU is presented. We used two DED-resistant genotypes, MDV2.3 and VAD2, a...
| Autores: | , , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/123684 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/123684 |
| Access Level: | acceso abierto |
| Palavra-chave: | 582.635.1 581.15 581.2 582.28 630*1 Botánica (Biología) Genética 2417 Biología Vegetal (Botánica) 2417.14 Genética Vegetal 3108 Fitopatología 3108.05 Hongos 3106 Ciencia Forestal |
| Resumo: | Dutch elm disease (DED), caused by Ophiostoma novo-ulmi (ONU), has devastated elm populations across Europe and North America since the 20th century. In this work, a de novo transcriptome assembly of Ulmus minor in response to ONU is presented. We used two DED-resistant genotypes, MDV2.3 and VAD2, and one DED-susceptible genotype, MDV1, to capture responses to ONU at four time points post-inoculation (6, 24, 72, and 144 hours). RNA from collected samples was isolated and sequenced producing 60.88 M 100 bp paired-end reads per sample. We performed a de novo transcriptome assembly combining data from the three genotypes. The assembly was functionally annotated and validated through differential gene expression analysis of the response. This dataset provides a valuable resource for studying molecular mechanisms of DED resistance in elms, contributing to broadening our understanding of tree immunity and facilitating potential applications in functional annotation of future genome assemblies. |
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