Interfacial water on collagen nanoribbons by 3D AFM

Collagen is the most abundant structural protein in mammals. Type I collagen in its fibril form has a characteristic pattern structure that alternates two regions called gap and overlap. The structure and properties of collagens are highly dependent on the water and mineral content of the environmen...

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Detalles Bibliográficos
Autores: Arvelo, Diana M., García Sacristán, Clara, Chacón, Enrique, Tarazona Lafarga, Pedro José, García, Ricardo
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/716439
Acceso en línea:http://hdl.handle.net/10486/716439
https://dx.doi.org/10.1063/5.0205611
Access Level:acceso abierto
Palabra clave:Collagen
structural protein
type I collagen
fibrillar structure
gap and overlap regions
properties of collagen
water and mineral content
3D AFM
structure of interfacial water
Física
Descripción
Sumario:Collagen is the most abundant structural protein in mammals. Type I collagen in its fibril form has a characteristic pattern structure that alternates two regions called gap and overlap. The structure and properties of collagens are highly dependent on the water and mineral content of the environment. Here, we apply 3D AFM to characterize at angstrom-scale resolution the interfacial water structure of collagen nanoribbons. For a neutral tip, the interfacial water structure is characterized by the oscillation of the water particle density distribution with a value of 0.3 nm (hydration layers). The interfacial structure does not depend on the collagen region. For a negatively charged tip, the interfacial structure might depend on the collagen region. Hydration layers are observed in overlap regions, while in gap regions, the interfacial solvent structure is dominated by electrostatic interactions. These interactions generate interlayer distances of 0.2 nm