Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants

Long-term treatments (April-June 2004) with 17α-methyltestosterone (MT) and/or human chorionic gonadotropin (hCG) were used to induce precocious sex change in 1-year-old juveniles (mean ± SD body weight of 131 ± 25 g) of the protogynous dusky grouper (Epinephelus marginatus). The MT treatment was gi...

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Autores: Sarter, Kerstin, Papadaki, Maria, Zanuy, Silvia, Mylonas, Constantinos
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/252184
Acceso en línea:http://hdl.handle.net/10261/252184
Access Level:acceso abierto
Palabra clave:Dusky grouper
Epinephelus
Sex inversion
Steroid implants
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spelling Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implantsSarter, KerstinPapadaki, MariaZanuy, SilviaMylonas, ConstantinosDusky grouperEpinephelusSex inversionSteroid implantsLong-term treatments (April-June 2004) with 17α-methyltestosterone (MT) and/or human chorionic gonadotropin (hCG) were used to induce precocious sex change in 1-year-old juveniles (mean ± SD body weight of 131 ± 25 g) of the protogynous dusky grouper (Epinephelus marginatus). The MT treatment was given once a month using controlled-release implants (1.5 mg per implant, ∼11 mg kg- 1 body weight), whereas hCG was injected intramuscularly once a month (MT/hCG group) or every 2 weeks (hCG group). In vitro mean (± SEM) daily and total MT release from the implants was 22.6 ± 5.4 μg day- 1 implant- 1 and 686 ± 113 μg implant- 1, respectively. In vivo, plasma MT levels of European sea bas (Dicentrarchus labrax) used as model fish (203 ± 41 g body weight) and given a single MT implant were 18.5 ± 0.5 ng ml- 1 one day after implantation, declined to 9.6 ± 2.7 ng ml- 1 by day 11 and remained stable between 7.9 and 5.5 ng ml- 1 until day 32. Plasma MT levels were significantly elevated (2-way ANOVA, P < 0.01) in dusky grouper juveniles in response to the MT treatment, both 6 and 12 weeks after beginning the treatment. On the contrary, no significant differences (2-way ANOVA, P > 0.05) between MT/hCG-treated and control fish were observed in plasma testosterone (T) or 11-keto T (11-KT). Pituitary luteinizing hormone (LH) content was significantly lower in both the MT/hCG and hCG groups, compared to controls (2-way ANOVA, P < 0.001), while plasma LH was below detection limits in all treatments. At the onset of the experiment all but one fish were already differentiated into females, having ovaries with primary oocytes. No signs of vitellogenesis or sex inversion were observed in fish from the control and hCG groups during the study. On the contrary, the MT implants induced complete sex change after 12 weeks, with the gonads organized in lobules and cysts filled with germ cells at all stages of spermatogenesis, including spermatozoa. This sex inversion was permanent and fish underwent complete spermatogenesis again during the following year. The results demonstrate that the produced MT-implants, but not the hCG injections, were highly efficient in inducing both sex inversion and complete spermatogenesis in pre-pubertal dusky grouper at the completion of their second year in life. Production of male fish at such an early age may dramatically reduce by many years the time required for natural puberty, sex inversion and the production of sperm by dusky grouper reared under culture conditions.Elsevier BVConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120062021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/252184reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.aquaculture.2006.01.034Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2521842026-05-22T06:33:51Z
dc.title.none.fl_str_mv Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
title Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
spellingShingle Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
Sarter, Kerstin
Dusky grouper
Epinephelus
Sex inversion
Steroid implants
title_short Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
title_full Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
title_fullStr Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
title_full_unstemmed Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
title_sort Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants
dc.creator.none.fl_str_mv Sarter, Kerstin
Papadaki, Maria
Zanuy, Silvia
Mylonas, Constantinos
author Sarter, Kerstin
author_facet Sarter, Kerstin
Papadaki, Maria
Zanuy, Silvia
Mylonas, Constantinos
author_role author
author2 Papadaki, Maria
Zanuy, Silvia
Mylonas, Constantinos
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Dusky grouper
Epinephelus
Sex inversion
Steroid implants
topic Dusky grouper
Epinephelus
Sex inversion
Steroid implants
description Long-term treatments (April-June 2004) with 17α-methyltestosterone (MT) and/or human chorionic gonadotropin (hCG) were used to induce precocious sex change in 1-year-old juveniles (mean ± SD body weight of 131 ± 25 g) of the protogynous dusky grouper (Epinephelus marginatus). The MT treatment was given once a month using controlled-release implants (1.5 mg per implant, ∼11 mg kg- 1 body weight), whereas hCG was injected intramuscularly once a month (MT/hCG group) or every 2 weeks (hCG group). In vitro mean (± SEM) daily and total MT release from the implants was 22.6 ± 5.4 μg day- 1 implant- 1 and 686 ± 113 μg implant- 1, respectively. In vivo, plasma MT levels of European sea bas (Dicentrarchus labrax) used as model fish (203 ± 41 g body weight) and given a single MT implant were 18.5 ± 0.5 ng ml- 1 one day after implantation, declined to 9.6 ± 2.7 ng ml- 1 by day 11 and remained stable between 7.9 and 5.5 ng ml- 1 until day 32. Plasma MT levels were significantly elevated (2-way ANOVA, P < 0.01) in dusky grouper juveniles in response to the MT treatment, both 6 and 12 weeks after beginning the treatment. On the contrary, no significant differences (2-way ANOVA, P > 0.05) between MT/hCG-treated and control fish were observed in plasma testosterone (T) or 11-keto T (11-KT). Pituitary luteinizing hormone (LH) content was significantly lower in both the MT/hCG and hCG groups, compared to controls (2-way ANOVA, P < 0.001), while plasma LH was below detection limits in all treatments. At the onset of the experiment all but one fish were already differentiated into females, having ovaries with primary oocytes. No signs of vitellogenesis or sex inversion were observed in fish from the control and hCG groups during the study. On the contrary, the MT implants induced complete sex change after 12 weeks, with the gonads organized in lobules and cysts filled with germ cells at all stages of spermatogenesis, including spermatozoa. This sex inversion was permanent and fish underwent complete spermatogenesis again during the following year. The results demonstrate that the produced MT-implants, but not the hCG injections, were highly efficient in inducing both sex inversion and complete spermatogenesis in pre-pubertal dusky grouper at the completion of their second year in life. Production of male fish at such an early age may dramatically reduce by many years the time required for natural puberty, sex inversion and the production of sperm by dusky grouper reared under culture conditions.
publishDate 2006
dc.date.none.fl_str_mv 2006
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/252184
url http://hdl.handle.net/10261/252184
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.aquaculture.2006.01.034
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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