Generation of a human iPSC line, IISHDOi002-A, with a 46, XY/47, XYY mosaicism and belonging to an African mitochondrial haplogroup

We have generated a human iPSC line, IISHDOi002-A, from commercial primary normal human dermal fibroblasts belonging to an African mitochondrial haplogroup (L3), and with a 46, XY/47, XYY mosaicism. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4 and cMyc were delivered using a non-integr...

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Detalles Bibliográficos
Autores: Ortuño-Costela, María del Carmen, Moreno-Izquierdo, Ana, Garesse Alarcón, Rafael, Gallardo, M. Esther
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/682534
Acceso en línea:http://hdl.handle.net/10486/682534
https://dx.doi.org/10.1016/j.scr.2018.02.009
Access Level:acceso abierto
Palabra clave:IISHDOi002-A
Dermal fibroblasts
African mitochondrial haplogroup
Factors Oct3/4, Sox2, Klf4 and cMyc
Sendai virus
Medicina
Descripción
Sumario:We have generated a human iPSC line, IISHDOi002-A, from commercial primary normal human dermal fibroblasts belonging to an African mitochondrial haplogroup (L3), and with a 46, XY/47, XYY mosaicism. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4 and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus