Molecular and morphological characterisation of Rotylenchus vitis n. sp. (Nematoda: Hoplolaimidae) infecting grapevine in southern Spain

Rotylenchus vitis n. sp., a new amphimictic species infesting soil and roots of commercial vineyards in southern Spain, is described. Rotylenchus vitis n. sp. is characterised by a truncate lip region with 7-9 annuli and continuous with the body contour, lateral fields areolated at pharyngeal region...

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Detalles Bibliográficos
Autores: Cantalapiedra-Navarrete, C., Liébanas, Gracia, Archidona-Yuste, Antonio, Palomares Rius, Juan E., Castillo, Pablo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/89925
Acceso en línea:http://hdl.handle.net/10261/89925
Access Level:acceso abierto
Palabra clave:D2-D3 region
Morphology
Morphometrics
New species
Phylogeny
Spiral nematodes
Taxonomy
Vitis vinifera
Descripción
Sumario:Rotylenchus vitis n. sp., a new amphimictic species infesting soil and roots of commercial vineyards in southern Spain, is described. Rotylenchus vitis n. sp. is characterised by a truncate lip region with 7-9 annuli and continuous with the body contour, lateral fields areolated at pharyngeal region only, body without longitudinal striations, stylet length of 36-48 οm, vulva position at 47-57%, tail rounded to hemispherical with 13-21 annuli. Morphologically, this species is related to R. cazorlaensis, R. capitatus, R. elegans, R. fabalus, R. iranicus, R. labiodiscus, R. montanus and R. troncapitatus. The results of the phylogenetic analysis based on the sequences of the D2-D3 expansion regions of the 28S and ITS1-rRNA genes confirmed the species differentiation and the close molecular relationship between R. eximius and R. montanus, respectively. © 2012 Koninklijke Brill NV, Leiden, The Netherlands.