In vitro antimicrobial activity of a new series of 1,4-naphthoquinones

The antibacterial activity of a series of 1,4-naphthoquinones was demonstrated. Disk diffusion tests were carried out against several Gram-positive and Gram-negative bacteria. The compound 5-amino- 8-hydroxy-1,4-naphthoquinone was the most effective, presenting inhibition zones measuring 20 mm again...

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Detalles Bibliográficos
Autores: Riffel, Alessandro, Medina, Luis Fernando da Costa, Stefani, Valter, Santos, R.C., Bizani, Delmar, Brandelli, Adriano
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2002
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/21273
Acceso en línea:http://hdl.handle.net/10183/21273
Access Level:acceso abierto
Palabra clave:Naftoquinonas
Atividade antimicrobiana
5-Amino-8-hydroxy-1,4- naphthoquinone
Antimicrobial agent
Staphylococcus aureus
Quinone
Naphthazarin
5-Acetamido-8-hydroxy-1,4-naphthoquinone
2,3-Diamino-1,4-naphthoquinone
Descripción
Sumario:The antibacterial activity of a series of 1,4-naphthoquinones was demonstrated. Disk diffusion tests were carried out against several Gram-positive and Gram-negative bacteria. The compound 5-amino- 8-hydroxy-1,4-naphthoquinone was the most effective, presenting inhibition zones measuring 20 mm against staphylococci, streptococci and bacilli at 50 μg/ml. Methicillin-resistant Staphylococcus aureus and several clinical isolates of this bacterium were also inhibited. Naphthazarin, 5-acetamido-8-hydroxy-1,4-naphthoquinone, and 2,3- diamino-1,4-naphthoquinone were the next most active compounds. The minimal inhibitory concentration of the active compounds was determined against S. aureus, ranging from 30 to 125 μg/ml. All compounds presented a minimal bactericidal concentration higher than 500 μg/ml, indicating that their effect was bacteriostatic. The EC50, defined as the drug concentration that produces 50% of maximal effect, was 8 μg/ml for 5-amino-8-hydroxy-1,4-naphthoquinone against S. aureus, S. intermedius, and S. epidermidis. These results indicate an effective in vitro activity of 5-amino-8-hydroxy-1,4-naphthoquinone and encourage further studies for its application in antibiotic therapy.