Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study

The aim of this study is to report the results of research work on the molecular mechanism of complex formation between chymotrypsin and a negatively charged natural strong polyelectrolyte, ι-carrageenan, using spectroscopy techniques. The carrageenan-chymotrypsin complex showed a maximal non-solubi...

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Detalles Bibliográficos
Autores: Woitovich Valetti, Nadia, Boeris, Valeria, Picó, Guillermo Alfredo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/197440
Acceso en línea:http://hdl.handle.net/11336/197440
Access Level:acceso abierto
Palabra clave:BTEE
CAR
CARRAGEENAN
CHY
CHYMOTRYPSIN
POLYELECTROLYTE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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network_name_str Argentina
repository_id_str
spelling Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability studyWoitovich Valetti, NadiaBoeris, ValeriaPicó, Guillermo AlfredoBTEECARCARRAGEENANCHYCHYMOTRYPSINPOLYELECTROLYTEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The aim of this study is to report the results of research work on the molecular mechanism of complex formation between chymotrypsin and a negatively charged natural strong polyelectrolyte, ι-carrageenan, using spectroscopy techniques. The carrageenan-chymotrypsin complex showed a maximal non-solubility at pH around 4.50 with a stoichiometric ratio between 8 and 33. g of chymotrypsin per g of carrageenan. These values were depended on the enzyme concentration, pH and ionic strength medium. The insoluble complex was redissolved by modifying the pH and by a NaCl concentration around 0.2. M in agreement with a coulombic mechanism of complex formation. The non-soluble complex formation showed biphasic kinetics. A fast step was carried out around 10. s and a coulombic mechanism takes place, and a slower step of around 120. s, where participate only Van der Waals forces. The enzymatic activity of chymotrypsin was maintained even in the presence of carrageenan (0.005%, w/v).Fil: Woitovich Valetti, Nadia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Boeris, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaElsevier Science2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197440Woitovich Valetti, Nadia; Boeris, Valeria; Picó, Guillermo Alfredo; Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study; Elsevier Science; International Journal of Biological Macromolecules; 52; 1; 10-2012; 45-510141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813012004114info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2012.10.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:37:21Zoai:ri.conicet.gov.ar:11336/197440instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:37:21.766CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
title Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
spellingShingle Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
Woitovich Valetti, Nadia
BTEE
CAR
CARRAGEENAN
CHY
CHYMOTRYPSIN
POLYELECTROLYTE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
title_full Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
title_fullStr Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
title_full_unstemmed Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
title_sort Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study
dc.creator.none.fl_str_mv Woitovich Valetti, Nadia
Boeris, Valeria
Picó, Guillermo Alfredo
author Woitovich Valetti, Nadia
author_facet Woitovich Valetti, Nadia
Boeris, Valeria
Picó, Guillermo Alfredo
author_role author
author2 Boeris, Valeria
Picó, Guillermo Alfredo
author2_role author
author
dc.subject.none.fl_str_mv BTEE
CAR
CARRAGEENAN
CHY
CHYMOTRYPSIN
POLYELECTROLYTE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic BTEE
CAR
CARRAGEENAN
CHY
CHYMOTRYPSIN
POLYELECTROLYTE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description The aim of this study is to report the results of research work on the molecular mechanism of complex formation between chymotrypsin and a negatively charged natural strong polyelectrolyte, ι-carrageenan, using spectroscopy techniques. The carrageenan-chymotrypsin complex showed a maximal non-solubility at pH around 4.50 with a stoichiometric ratio between 8 and 33. g of chymotrypsin per g of carrageenan. These values were depended on the enzyme concentration, pH and ionic strength medium. The insoluble complex was redissolved by modifying the pH and by a NaCl concentration around 0.2. M in agreement with a coulombic mechanism of complex formation. The non-soluble complex formation showed biphasic kinetics. A fast step was carried out around 10. s and a coulombic mechanism takes place, and a slower step of around 120. s, where participate only Van der Waals forces. The enzymatic activity of chymotrypsin was maintained even in the presence of carrageenan (0.005%, w/v).
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197440
Woitovich Valetti, Nadia; Boeris, Valeria; Picó, Guillermo Alfredo; Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study; Elsevier Science; International Journal of Biological Macromolecules; 52; 1; 10-2012; 45-51
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197440
identifier_str_mv Woitovich Valetti, Nadia; Boeris, Valeria; Picó, Guillermo Alfredo; Characterization of chymotrypsin-ι-carrageenan complex in aqueous solution: A solubility and thermodynamical stability study; Elsevier Science; International Journal of Biological Macromolecules; 52; 1; 10-2012; 45-51
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813012004114
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2012.10.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,81155

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