Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h i...
| Authors: | , , , |
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| Format: | article |
| Status: | Versión enviada para evaluación y publicación |
| Publication Date: | 1997 |
| Country: | Argentina |
| Institution: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repository: | CIC Digital (CICBA) |
| Language: | Spanish |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/4899 |
| Online Access: | https://digital.cic.gba.gob.ar/handle/11746/4899 |
| Access Level: | Open access |
| Keyword: | Ciencias Químicas advanced glycation endproducts diabetes mellitus osteoblastic cells cell proliferation cell differentiation bone |
| Summary: | Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner. |
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